{"id":5534,"date":"2020-12-23T09:38:05","date_gmt":"2020-12-23T09:38:05","guid":{"rendered":"https:\/\/www.gyanvihar.org\/journals\/?p=5534"},"modified":"2021-01-05T06:48:20","modified_gmt":"2021-01-05T06:48:20","slug":"pharmacognostic-study-of-bauhinia-acuminata-linn","status":"publish","type":"post","link":"https:\/\/www.gyanvihar.org\/journals\/pharmacognostic-study-of-bauhinia-acuminata-linn\/","title":{"rendered":"Pharmacognostic Study of Bauhinia Acuminata Linn."},"content":{"rendered":"<p style=\"text-align: justify\"><strong><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Mohsin Jahangeer Jamadar*<sup>1<\/sup>, Preeti\u00a0 Khulbe<sup>1<\/sup> &amp; Shrinivas. K. Mohite<sup>2<\/sup><\/span><\/strong><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><sup>1<\/sup> School of Pharmacy, Suresh Gyan Vihar University, Mahal, Jagatpura, Jaipur, 302017<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><sup>2 <\/sup>Rajarambapu College of Pharmacy, Kasegaon, Taluka &#8211; Walwa, Sangli, Maharashtra<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>ABSTRACT<\/strong><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Aim: <\/strong>To study pharmacognostic and phytochemical standards of <em>Bauhinia acuminata<\/em> Linn<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Method:<\/strong> The leaves of <em>Bauhinia acuminata<\/em> Linn. Were studied by Microscopic parameter, physicochemical parameter and phytochemical parameter.<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Result:<\/strong> The microscopic study leaf of <em>Bauhinia acuminata<\/em> Linn showed presence of xylem &amp; phloem upper epidermis, lower epidermis and stomata. The powder studies of leaves of <em>Bauhinia acuminata<\/em> Linn showed the presence of Trichomes, Xylem &amp; Phloem, Stomata and Ca- oxalate crystals. Physicochemical parameters such as ash value, acid insoluble value, moisture content and alcohol soluble and water soluble extractive value were determined. The petroleum ether extract of <em>Bauhinia acuminata<\/em> Linn showed the presence of phenolic compound &amp;flavonoids while chloroform extract showed the presence of Glycoside, flavonoids and phytosterol. The ethyl acetate extract of <em>Bauhinia acuminata<\/em> Linn showed the presence of phytosterol and flavonoids and ethanolic extract showed the presence of Glycoside, flavonoids, saponins and phenolic compound. The aqueous extract of <em>Bauhinia acuminata<\/em> Linn showed the presence of phytosterol, flavonoids, saponins and phenolic compound.<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Conclusion:<\/strong> The results of the studies can provide standards for <em>Bauhinia acuminata<\/em> Linn.<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>INTRODUCTION:<\/strong><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">The standardization of herbal drug involve selection and handling of crude material\u00a0 and more attention given towards macroscopic, microscopic examination, extract value, chemical evaluation, ash values, moisture content and different physicochemical parameter.<sup>(1,2)<\/sup> Phytochemicals and pharmacologically active compounds are found in plants by primary and secondary metabolism which give health benefits other than macronutrients and micronutrients <sup>(3).<\/sup> Phytochemicals contribute to the plants colour, aroma, flavour and protect plants from various disease, environmental hazards, stress, UV exposure and pollutions<sup>.(4,5).<\/sup>The phytochemicals are also having role in human health when their dietary intake is significant. More than 4,000 phytochemicals are identify by physical and chemical characterization. <sup>(6)<\/sup> And about 150 phytochemicals have been studied in detail. These phytochemicals are present in vegetable, fruits, legumes, nuts, whole seeds, fungi, grains, species and herbs <sup>(7). <\/sup>And also found in Broccoli, cherries, tomatoes, beans, garlic, carrots, grapes, cabbage, strawberries, raspberries, onions, whole wheat bread, and soy foods. <sup>(8).<\/sup> These phytochemicals present in different parts of the plants, such as in the stems, roots, leaves, fruits, flowers, or seeds <sup>(9).<\/sup><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><em>Bauhinia acuminata<\/em> Linn belong to family Caesalpiniaceae and native to Asia. The plant will grow up to 3 meter height. It consist white flowers cover this tree in spring and have sweet clean fragrance. Sometimes it is called Snowy Orchid Tree because of white flowers look like snowflakes hanging on the branches. The leaves are of cow&#8217;s hoof shaped. The <em>Bauhinia acuminata<\/em> Linn consist of flavonoids, alkaloids, glycoside, Saponin, tannin, and steroids. The Indian vaiydas recommended the leaves and bark of <em>Bauhinia acuminata <\/em>Linn for the treatment of biliousness. In Malaysia and Indonesia the plant is used for common cold and cough treatment. <sup>(10)<\/sup>.<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">This study is designed to various pharmacognostic and phytochemical standards of <em>Bauhinia acuminata<\/em> Linn which will ensure the safety, purity, and efficacy of this medicinal plant.<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>METHODS<\/strong><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Collection and preparation of plant material: The fresh leaves of <em>Bauhinia acuminata<\/em> Linn used in the study, the plants are collected from Kolhapur, Maharashtra state, and authenticated by Botanical Survey of India, Pune, and Maharashtra. (BSI\/WRC\/IDEN.CER.\/2019\/H3 dated19\/08\/2019)<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Preparation of Extract: <\/strong>The leaves of plants were separated dried. After drying leaves were exposed to size reduction. The powdered leaves (500 g) were undergone to Soxhlet extraction with different solvent to get Petroleum ether extract (PE),Chloroform extract (CH),Ethyl acetate extract(EA) ,Ethanol extract(ET),Aqueous extract (Aq).<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Microscopic Studies of Plants<\/strong><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Thinnest possible section of leaf (through midrib to study nature of epidermis, trichomes and stomata) were taken on a slide, mounted in a solution of chloral hydrate and warmed slightly to decolorize. To study microscopic examination of powdered drug, powder samples were taken on a slide and add few drops of chloral hydrate heated for 1-2 minutes and viewed under the microscope after mounting it on a glass slide using glycerine and covering with a cover slip.\u00a0 For observing\u00a0 Lignified tissues, powder first treated with chloral hydrate and then add few drops of 1:1 Phloroglucinol and conc. Hcl after 3- 4 minutes, mounted in glycerine. Pink stained are observed for Lignified tissues. <sup>(11, 12, 13)<\/sup>.<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Physicochemical Evaluation<\/strong><\/span><\/p>\n<ol style=\"text-align: justify\">\n<li><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong> Determination of Ash values<\/strong><\/span><\/li>\n<\/ol>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">2g of the air-dried leaf powder of <em>Bauhinia acuminata<\/em> Linn taken in separate a tared silica dish and incinerate at a high temperature about 450\u00b0c until it is free from carbon, cool and take a weight.<\/span><\/p>\n<ol style=\"text-align: justify\" start=\"2\">\n<li><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong> Acid insoluble ash<\/strong><\/span><\/li>\n<\/ol>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Ash boiled for five minute with 25 ml of 1 N HCl. It was filtered through an ashless filter paper to collect the insoluble residue. The residue obtained was washed with hot water and was further ignited, cooled and weighed. The percentage of acid insoluble ash was calculated with reference to air-dried sample of the drug.<\/span><\/p>\n<ol style=\"text-align: justify\" start=\"3\">\n<li><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong> Moisture Content<\/strong><\/span><\/li>\n<\/ol>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Take container with lid and clean it.\u00a0 Dry the container and note down its weight (W1). Take a powder of <em>Bauhinia acuminata<\/em> Linn in the container and weigh with lid (W2). Keep the container in oven and removed the lid. Dry the sample to constant weight maintaining the temperature between 105<sup>0<\/sup>C to 110<sup>0<\/sup>C for a period usually 16 to 24 hours.\u00a0 Finally record the constant weight (W3).<\/span><\/p>\n<ol style=\"text-align: justify\" start=\"4\">\n<li><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong> Water soluble extractive values<\/strong><\/span><\/li>\n<\/ol>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">5 g powdered drug was weighed and transferred in a conical flask and macerated with 100 ml water. After maceration, the content of the flask was filtered. 25 ml of the filtrate was placed in a weighed porcelain dish and evaporated to dryness. Further weight difference was calculated and extractive values were expressed in % (w\/w).<\/span><\/p>\n<ol style=\"text-align: justify\" start=\"5\">\n<li><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong> Alcohol soluble extractive values<\/strong><\/span><\/li>\n<\/ol>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">5 g powdered drug was weighed and transferred in a conical flask. It was macerated with 100 ml alcohol. After maceration, the content of the flask was filtered. 25 ml of the filtrate was kept in a weighed porcelain dish and evaporated to dryness. Further weight difference was calculated and extractive values were expressed in % (w\/w).<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Phytochemical Investigation<\/strong><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Phytochemical test on different extract were performed according to Khandelwal K. Practical Pharmacognosy book. <sup>(11)<\/sup><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>RESULT<\/strong><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Microscopic Studies of Plants<\/strong><\/span><\/p>\n<p><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-5519 \" src=\"https:\/\/www.gyanvihar.org\/journals\/wp-content\/uploads\/2020\/12\/2.1.jpg\" alt=\"\" width=\"449\" height=\"336\" srcset=\"https:\/\/www.gyanvihar.org\/journals\/uploads\/2020\/12\/2.1.jpg 635w, https:\/\/www.gyanvihar.org\/journals\/uploads\/2020\/12\/2.1-624x467.jpg 624w\" sizes=\"auto, (max-width: 449px) 100vw, 449px\" \/><\/span><\/p>\n<p style=\"text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Figure 1: T.S of <em>Bauhinia acuminata<\/em> Linn leaf<\/strong><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>\u00a0<\/strong><strong>\u00a0<\/strong><strong>Powder characteristics of <em>Bauhinia acuminata<\/em> Linn.<\/strong><\/span><\/p>\n<p><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-5521 alignleft\" src=\"https:\/\/www.gyanvihar.org\/journals\/wp-content\/uploads\/2020\/12\/2.2-e1608714246174.png\" alt=\"\" width=\"337\" height=\"253\" srcset=\"https:\/\/www.gyanvihar.org\/journals\/uploads\/2020\/12\/2.2-e1608714246174.png 640w, https:\/\/www.gyanvihar.org\/journals\/uploads\/2020\/12\/2.2-e1608714246174-624x468.png 624w\" sizes=\"auto, (max-width: 337px) 100vw, 337px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-5522 \" src=\"https:\/\/www.gyanvihar.org\/journals\/wp-content\/uploads\/2020\/12\/2.3.jpg\" alt=\"\" width=\"329\" height=\"247\" srcset=\"https:\/\/www.gyanvihar.org\/journals\/uploads\/2020\/12\/2.3.jpg 640w, https:\/\/www.gyanvihar.org\/journals\/uploads\/2020\/12\/2.3-624x468.jpg 624w\" sizes=\"auto, (max-width: 329px) 100vw, 329px\" \/><\/span><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Figure 2:\u00a0 Lignified cell\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0Figure 3:\u00a0 Calcium oxalate<\/strong><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-5523 alignleft\" src=\"https:\/\/www.gyanvihar.org\/journals\/wp-content\/uploads\/2020\/12\/2.4.jpg\" alt=\"\" width=\"261\" height=\"196\" srcset=\"https:\/\/www.gyanvihar.org\/journals\/uploads\/2020\/12\/2.4.jpg 640w, https:\/\/www.gyanvihar.org\/journals\/uploads\/2020\/12\/2.4-624x468.jpg 624w\" sizes=\"auto, (max-width: 261px) 100vw, 261px\" \/> <img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-5524 \" src=\"https:\/\/www.gyanvihar.org\/journals\/wp-content\/uploads\/2020\/12\/2.5.jpg\" alt=\"\" width=\"439\" height=\"205\" \/>\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 <strong>Figure 4: Trichomes\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0Figure 5: Lignified bordered pitted<\/strong><\/span><\/p>\n<p><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-5525 \" src=\"https:\/\/www.gyanvihar.org\/journals\/wp-content\/uploads\/2020\/12\/2.6.jpg\" alt=\"\" width=\"468\" height=\"287\" \/><\/span><\/p>\n<p style=\"text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Figure 6: Phloem<\/strong><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>\u00a0<\/strong><\/span><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Physicochemical studies<\/strong><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Table-1. Physical Parameters of the Leaves of <em>Bauhinia acuminata <\/em>Linn.<\/strong><\/span><\/p>\n<table style=\"border-collapse: collapse;width: 79.1263%;height: 194px\" border=\"1\">\n<tbody>\n<tr>\n<td style=\"width: 15.8757%;text-align: center\" width=\"85\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Sr. No<\/strong><\/span><\/td>\n<td style=\"width: 55.1905%;text-align: center\" width=\"288\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Parameters<\/strong><\/span><\/td>\n<td style=\"width: 40.7474%;text-align: center\" width=\"216\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Result (%)<\/strong><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 15.8757%;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">1<\/span><\/td>\n<td style=\"width: 55.1905%;text-align: center\" width=\"288\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Ash value<\/span><\/td>\n<td style=\"width: 40.7474%;text-align: center\" width=\"216\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">10<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 15.8757%;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">2<\/span><\/td>\n<td style=\"width: 55.1905%;text-align: center\" width=\"288\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Acid insoluble value<\/span><\/td>\n<td style=\"width: 40.7474%;text-align: center\" width=\"216\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">0.9<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 15.8757%;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">3<\/span><\/td>\n<td style=\"width: 55.1905%;text-align: center\" width=\"288\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">moisture content<\/span><\/td>\n<td style=\"width: 40.7474%;text-align: center\" width=\"216\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">8.5<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 15.8757%;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">4<\/span><\/td>\n<td style=\"width: 55.1905%;text-align: center\" width=\"288\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Extractive value(water soluble)<\/span><\/p>\n<p><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">(Alcohol soluble)<\/span><\/td>\n<td style=\"width: 40.7474%;text-align: center\" width=\"216\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">20.2<\/span><\/p>\n<p><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">6.3<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>\u00a0<\/strong><\/span><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Phytochemical Investigation<\/strong><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Table-2: Preliminary phytochemical screening of various extracts of <em>Bauhinia acuminata<\/em> Linn.<\/strong><\/span><\/p>\n<table style=\"border-collapse: collapse;width: 76.0922%;height: 900px\" border=\"1\">\n<tbody>\n<tr style=\"height: 48px\">\n<td style=\"width: 20.7494%;height: 48px;text-align: center\" width=\"169\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Chemical Constituents<\/strong><\/span><\/td>\n<td style=\"width: 19.2145%;height: 48px;text-align: center\" width=\"162\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>Tests<\/strong><\/span><\/td>\n<td style=\"width: 11.5243%;height: 48px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>PE<\/strong><\/span><\/td>\n<td style=\"width: 10.7333%;height: 48px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>CH<\/strong><\/span><\/td>\n<td style=\"width: 11.1976%;height: 48px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>EA<\/strong><\/span><\/td>\n<td style=\"width: 10.4955%;height: 48px;text-align: center\" width=\"72\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>ET<\/strong><\/span><\/td>\n<td style=\"width: 12.5264%;height: 48px;text-align: center\" width=\"66\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>AQ<\/strong><\/span><\/td>\n<\/tr>\n<tr style=\"height: 24px\">\n<td style=\"width: 20.7494%;height: 72px;text-align: center\" rowspan=\"3\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Alkaloids<\/span><\/td>\n<td style=\"width: 19.2145%;height: 24px;text-align: center\" width=\"162\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Mayer\u2019s<\/span><\/td>\n<td style=\"width: 11.5243%;height: 24px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">\u02d7<\/span><\/td>\n<td style=\"width: 10.7333%;height: 24px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 11.1976%;height: 24px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 10.4955%;height: 24px;text-align: center\" width=\"72\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<td style=\"width: 12.5264%;height: 24px;text-align: center\" width=\"66\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<\/tr>\n<tr style=\"height: 24px\">\n<td style=\"width: 19.2145%;height: 24px;text-align: center\" width=\"162\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Wagner\u2019s<\/span><\/td>\n<td style=\"width: 11.5243%;height: 24px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 10.7333%;height: 24px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 11.1976%;height: 24px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 10.4955%;height: 24px;text-align: center\" width=\"72\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 12.5264%;height: 24px;text-align: center\" width=\"66\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<\/tr>\n<tr style=\"height: 24px\">\n<td style=\"width: 19.2145%;height: 24px;text-align: center\" width=\"162\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Dragendroff\u2019s<\/span><\/td>\n<td style=\"width: 11.5243%;height: 24px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 10.7333%;height: 24px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 11.1976%;height: 24px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 10.4955%;height: 24px;text-align: center\" width=\"72\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 12.5264%;height: 24px;text-align: center\" width=\"66\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<\/tr>\n<tr style=\"height: 24px\">\n<td style=\"width: 20.7494%;height: 98px;text-align: center\" rowspan=\"3\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Flavonoids<\/span><\/td>\n<td style=\"width: 19.2145%;height: 24px;text-align: center\" width=\"162\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Shinado<\/span><\/td>\n<td style=\"width: 11.5243%;height: 24px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<td style=\"width: 10.7333%;height: 24px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">++<\/span><\/td>\n<td style=\"width: 11.1976%;height: 24px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+ +<\/span><\/td>\n<td style=\"width: 10.4955%;height: 24px;text-align: center\" width=\"72\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+++<\/span><\/td>\n<td style=\"width: 12.5264%;height: 24px;text-align: center\" width=\"66\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<\/tr>\n<tr style=\"height: 26px\">\n<td style=\"width: 19.2145%;height: 26px;text-align: center\" width=\"162\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Lead acetate<\/span><\/td>\n<td style=\"width: 11.5243%;height: 26px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">++<\/span><\/td>\n<td style=\"width: 10.7333%;height: 26px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<td style=\"width: 11.1976%;height: 26px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<td style=\"width: 10.4955%;height: 26px;text-align: center\" width=\"72\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><sub>++<\/sub><\/span><\/td>\n<td style=\"width: 12.5264%;height: 26px;text-align: center\" width=\"66\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+++<\/span><\/td>\n<\/tr>\n<tr style=\"height: 48px\">\n<td style=\"width: 19.2145%;height: 48px;text-align: center\" width=\"162\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Alkaline reagents<\/span><\/td>\n<td style=\"width: 11.5243%;height: 48px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<td style=\"width: 10.7333%;height: 48px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<td style=\"width: 11.1976%;height: 48px;text-align: center\" width=\"60\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+++<\/span><\/td>\n<td style=\"width: 10.4955%;height: 48px;text-align: center\" width=\"72\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">++<\/span><\/td>\n<td style=\"width: 12.5264%;height: 48px;text-align: center\" width=\"66\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">++<\/span><\/td>\n<\/tr>\n<tr style=\"height: 24px\">\n<td style=\"width: 20.7494%;height: 128px;text-align: center\" rowspan=\"3\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Carbohydrates<\/span><\/td>\n<td style=\"width: 19.2145%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Molisch\u2019s<\/span><\/td>\n<td style=\"width: 11.5243%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<td style=\"width: 10.7333%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 11.1976%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 10.4955%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 12.5264%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<\/tr>\n<tr style=\"height: 56px\">\n<td style=\"width: 19.2145%;height: 56px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Barfoed\u2019s<\/span><\/td>\n<td style=\"width: 11.5243%;height: 56px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 10.7333%;height: 56px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 11.1976%;height: 56px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<td style=\"width: 10.4955%;height: 56px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 12.5264%;height: 56px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<\/tr>\n<tr style=\"height: 48px\">\n<td style=\"width: 19.2145%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Libermann Burchard\u2019s<\/span><\/td>\n<td style=\"width: 11.5243%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 10.7333%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 11.1976%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 10.4955%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<td style=\"width: 12.5264%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<\/tr>\n<tr style=\"height: 24px\">\n<td style=\"width: 20.7494%;height: 72px;text-align: center\" rowspan=\"2\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Phytosterols and Triterpinoids.<\/span><\/td>\n<td style=\"width: 19.2145%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Salkowaski<\/span><\/td>\n<td style=\"width: 11.5243%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 10.7333%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">++<\/span><\/td>\n<td style=\"width: 11.1976%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">++<\/span><\/td>\n<td style=\"width: 10.4955%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 12.5264%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<\/tr>\n<tr style=\"height: 48px\">\n<td style=\"width: 19.2145%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Libermann Burchard\u2019s<\/span><\/td>\n<td style=\"width: 11.5243%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 10.7333%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<td style=\"width: 11.1976%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+++<\/span><\/td>\n<td style=\"width: 10.4955%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<td style=\"width: 12.5264%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">++<\/span><\/td>\n<\/tr>\n<tr style=\"height: 24px\">\n<td style=\"width: 20.7494%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Glycosides<\/span><\/td>\n<td style=\"width: 19.2145%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Legal\u2019s<\/span><\/td>\n<td style=\"width: 11.5243%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">\u02d7<\/span><\/td>\n<td style=\"width: 10.7333%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">++<\/span><\/td>\n<td style=\"width: 11.1976%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">\u02d7<\/span><\/td>\n<td style=\"width: 10.4955%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">++<\/span><\/td>\n<td style=\"width: 12.5264%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">\u02d7<\/span><\/td>\n<\/tr>\n<tr style=\"height: 24px\">\n<td style=\"width: 20.7494%;height: 24px;text-align: center\"><\/td>\n<td style=\"width: 19.2145%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Borntrager\u2019s<\/span><\/td>\n<td style=\"width: 11.5243%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 10.7333%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<td style=\"width: 11.1976%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 10.4955%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 12.5264%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<\/tr>\n<tr style=\"height: 48px\">\n<td style=\"width: 20.7494%;height: 48px;text-align: center\"><\/td>\n<td style=\"width: 19.2145%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Liebermann Burchard<\/span><\/td>\n<td style=\"width: 11.5243%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 10.7333%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">++<\/span><\/td>\n<td style=\"width: 11.1976%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<td style=\"width: 10.4955%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<td style=\"width: 12.5264%;height: 48px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<\/tr>\n<tr style=\"height: 24px\">\n<td style=\"width: 20.7494%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Saponins<\/span><\/td>\n<td style=\"width: 19.2145%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Foam or Froth<\/span><\/td>\n<td style=\"width: 11.5243%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 10.7333%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<td style=\"width: 11.1976%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">&#8211;<\/span><\/td>\n<td style=\"width: 10.4955%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">++<\/span><\/td>\n<td style=\"width: 12.5264%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">+<\/span><\/td>\n<\/tr>\n<tr style=\"height: 24px\">\n<td style=\"width: 20.7494%;height: 24px;text-align: center\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Tannins and Phenolic Compounds<\/span><\/td>\n<td style=\"width: 19.2145%;text-align: center\">5%FeCl3<\/p>\n<p>Lead Acetate<\/p>\n<p>solution<\/p>\n<p>Gelatin Solution<\/p>\n<p>Bromine Water<\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">Acetic Acid<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">Potassium Dichromate<\/span><\/td>\n<td style=\"width: 11.5243%;text-align: center\"><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">+<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">++<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">+<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">+<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">++<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">+<\/span><\/td>\n<td style=\"width: 10.7333%;text-align: center\"><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">&#8211;<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">&#8211;<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">&#8211;<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">&#8211;<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">&#8211;<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">&#8211;<\/span><\/td>\n<td style=\"width: 11.1976%;text-align: center\"><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">&#8211;<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">&#8211;<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">&#8211;<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">&#8211;<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">&#8211;<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">&#8211;<\/span><\/p>\n<p>&nbsp;<\/td>\n<td style=\"width: 10.4955%;text-align: center\"><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">+<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">+<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">+<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">++<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">&#8211;<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">&#8211;<\/span><\/p>\n<p>&nbsp;<\/td>\n<td style=\"width: 12.5264%;text-align: center\"><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">&#8211;<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">++<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">&#8211;<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">+<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">+<\/span><\/p>\n<p><span style=\"font-size: 12pt;font-family: 'times new roman', times, serif\">+<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Abbreviations: PE:- Petroleum ether extract, CH:- Chloroform extract , EA:- Ethyl acetate extract , <\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">ET:- Ethanol extract, <\/span><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Aq:- Aqueous extract.<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">(+++) Appreciable amount; (++) Moderate amount; (+) Trace amount; (-) completely absent<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>\u00a0<\/strong><\/span><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>DISCUSSIONS<\/strong><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">The pharmacognostic study established the diagnostic characteristics of <em>Bauhinia acuminata<\/em> Linn. Which is helpful for quality control measures to ensure the quality, safety and efficacy.The different pharmacognostic parameters studied here are helpful to identify and authenticate the <em>Bauhinia acuminata<\/em> Linn and this will prove useful in the preparation of herbal monographs. (14) Most herbal preparations available in market are easily adulterated or substituted. The application pharmacognostic parameter like microscopy, macroscopy, phytochemical analysis, physicochemical parameter would be helpful for identification of herbal plant. The microscopical character like epidermal cell, stomata, vein termination number and palisade ration widely recognized in taxonomic considerations and used in the identification of taxa at genus as well as species levels. <sup>(15,16).<\/sup> The microscopic study of leaf of <em>Bauhinia acuminata<\/em> Linn showed presence of phloem and xylem, upper epidermis, lower epidermis and stomata. The powder studies of leaves of <em>Bauhinia acuminata<\/em> Linn showed the presence of Trichomes Xylem &amp; Phloem, Stomata and Ca- oxalate crystals.<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">The physicochemical parameters are constant for a plant hence it is useful for settings standards for crude drug. Various physicochemical parameters like, acid insoluble value, ash value, moisture content and alcohol soluble and water soluble extractive value were determined. These parameters are important for detection of drug improper handling or adulteration of raw materials. The ash value, indicate \u00a0inorganic composition and impurities in a plant drug<sup>.(1)<\/sup> The moisture content gives the information of stability of crude drug. The phytochemical study is important parameter which will give an indication of pharmacological activities. The petroleum ether extract of <em>Bauhinia acuminata<\/em> Linn showed the presence of\u00a0 \u00a0flavonoids and phenolic compound while chloroform extract showed the presence of glycoside, flavonoids and phytosterols. The ethyl acetate extract of <em>Bauhinia acuminata<\/em> Linn showed the presence of phytosterols and flavonoids and ethanolic extract showed the presence of Glycoside, flavonoids, saponins and phenolic compound. The aqueous extract of <em>Bauhinia acuminata<\/em> Linn showed the presence of phytosterols, flavonoids, saponins and phenolic compound.<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>CONCLUSIONS<\/strong><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">The pharmacognostic studies of <em>Bauhinia acuminata<\/em> Linn is carried by various parameters. The information gather by this study will help in identification and authentication of <em>Bauhinia acuminata<\/em> Linn.<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>ACKNOWLEDGEMENT<\/strong><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">We thank Appasaheb Birnale College of pharmacy for providing facilities for research work. And also thank Suresh Gyan Vihar University, Jaipur.<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>DISCLOSURE<\/strong><\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Author declares that he has no conflict of interest. And also this article does not contain any studies with human or animal subjects.<\/span><\/p>\n<p style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\"><strong>BIBLOGRAPHY<\/strong><\/span><\/p>\n<ol>\n<li style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Geneva: WHO; 1996. WHO. Quality Assurance Pharmaceuticals: A Compendium of Guidelines and Related Materials, Good Manufacturing Practices and Inspection; p. 2. [Google Scholar]<\/span><\/li>\n<li style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">WHO Technical Report Series. Geneva: WHO; 1996. WHO. Guidelines for the Assessment of Herbal Medicines; p. 863<\/span><\/li>\n<li style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Reyad-Ul-Ferdous, et.al. Ex-Vivo Anti-Inflammatory and Antimicrobial Activities of the Leaves of Bauhinia acuminata: American Journal of Life Sciences.2014; 2(5): 267-270.<\/span><\/li>\n<li style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Sharma r. k, goyal a. k and bhat r. a. Antifertility activity of plants extracts on female reproduction: International journal of pharmacy and biological sciences.2013; 3(3): 493-514.<\/span><\/li>\n<li style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Mohamed Shaheen, Mamdouh El-Gamal. Antiviral activity of Bauhinia variegata extracts against rotavirus in vitro: Current Science International. 2014, 3(3): 172-178.<\/span><\/li>\n<li style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Kanchan Lata Singh, D. K. Singh and Vinay Kumar Singh. 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Manipal, India: Manipal Press Ltd; 2006.<\/span><\/li>\n<li style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Iyengar MA. Pharmacognosy of Powdered Crude Drugs, 8th ed. Manipal, India: Manipal Press Ltd; 2007.<\/span><\/li>\n<li style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Nayak BS, Patel KN. Pharmacognostic studies of the Jatrophacurcas leaves. Int J PharmTech Res. 2010;2:140\u20133.<\/span><\/li>\n<li style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Rao RS, Ramayya N. Trichome types and their taxonomic importance in the Tiliaceae. Indian J Bot. 1987;10:65\u201373.<\/span><\/li>\n<li style=\"text-align: justify\"><span style=\"font-family: 'times new roman', times, serif;font-size: 12pt\">Trease GE, Evans WC. 12th ed. London: English Language Book Society\/Bailliere Tindall; 1983. Pharmacognosy<\/span><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Mohsin Jahangeer Jamadar*1, Preeti\u00a0 Khulbe1 &amp; Shrinivas. K. Mohite2 1 School of Pharmacy, Suresh Gyan Vihar University, Mahal, Jagatpura, Jaipur, 302017 2 Rajarambapu College of Pharmacy, Kasegaon, Taluka &#8211; Walwa, Sangli, Maharashtra ABSTRACT Aim: To study pharmacognostic and phytochemical standards of Bauhinia acuminata Linn Method: The leaves of Bauhinia acuminata Linn. Were studied by Microscopic [&hellip;]<\/p>\n","protected":false},"author":6,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[315,321],"tags":[],"class_list":["post-5534","post","type-post","status-publish","format-standard","hentry","category-suresh-gyan-vihar-university-journal-of-pharmaceutical-research-and-education","category-volume-5-issue-2-2020"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.7 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>research journal - Research Journal<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.gyanvihar.org\/journals\/pharmacognostic-study-of-bauhinia-acuminata-linn\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Pharmacognostic Study of Bauhinia Acuminata Linn. - research journal\" \/>\n<meta property=\"og:description\" content=\"Mohsin Jahangeer Jamadar*1, Preeti\u00a0 Khulbe1 &amp; Shrinivas. 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K. Mohite2 1 School of Pharmacy, Suresh Gyan Vihar University, Mahal, Jagatpura, Jaipur, 302017 2 Rajarambapu College of Pharmacy, Kasegaon, Taluka &#8211; Walwa, Sangli, Maharashtra ABSTRACT Aim: To study pharmacognostic and phytochemical standards of Bauhinia acuminata Linn Method: The leaves of Bauhinia acuminata Linn. 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